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作者对急性白血病患儿在完全缓解期中,用具有“活力”的同种异体白血病细胞作主动免疫治疗,并进行了观察。选择急性白血病的急性期,白细胞增高(50.000~160,000)、白血病细胞占50~80%的患儿,从其外周血液中分离出有活力的白血病细胞,浓集于少量血浆中。分装安瓿中封口后,—196°冻存。临用时混匀,肌肉注射剂量一般为0.6~3.0毫升中含有白血病细胞3×10~8~1.5×10~9。有1例通过骨髓穿刺取自另一急性白血病患者的骨髓液2~5毫升(2.5×10~9
The author of acute leukemia in children with complete remission, with “viable” allogenic leukemia cells for active immunotherapy, and were observed. Select the acute phase of acute leukemia, leukocytosis (50.000 ~ 160,000), leukemia cells accounted for 50 to 80% of children isolated from the peripheral blood of viable leukemia cells concentrated in a small amount of plasma. After the ampoule was sealed in ampoules, it was frozen at -196 ° C. Pro-mix, the intramuscular dose is generally 0.6 to 3.0 ml contains leukemia cells 3 × 10 ~ 8 ~ 1.5 × 10 ~ 9. There was 1 case of bone marrow fluid taken from another patient with acute leukemia by bone marrow puncture 2 to 5 ml (2.5 × 10 ~ 9