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目的探讨中国汉族人群rs11970286多态性与QT间期及室性心动过速(室速)的关系。方法 242例室速患者为室速组,1 002例健康人群为对照组;2组均行心电图检查,测量心率、PR间期、QRS时限、QT间期及QTc间期。提取2组外周血DNA,采用Rotor-Gene TM6000高分辨率溶解曲线对rs11970286进行基因分型,采用基因学软件PLINK v1.05对rs11970286多态性与QT间期、室速、特发性室速的发生进行关联分析。结果室速组患者合并高血压、糖尿病比率(30.99%、11.57%)高于对照组(5.49%、0.80%)(P<0.05),QT间期[(380.72±58.82)ms]与对照组[(376.71±27.92)ms]比较差异无统计学意义(P>0.05);所有研究对象中rs11970286T等位基因与QT间期呈负相关(β=-14.071,P=0.018),但与校正后的QTc无明显相关性(β=-16.876,P=0.062);室速组rs11970286基因型与等位基因分布频率与对照组比较差异均无统计学意义(P>0.05);特发性室速53例(特发性室速组)rs11970286CT基因型(16.981%)和TT+CT(显性基因模式)基因型(22.642%)分布频率低于对照组(39.521%、48.204%)(P<0.05),TT基因型分布频率(5.660%)与对照组(8.682%)比较差异无统计学意义(P>0.05),T等位基因分布频率(14.151%)低于对照组(28.443%)(P<0.01),T等位基因可能是特发性室速的保护因素(OR=0.415,95%CI:1.385~4.199,P<0.001);rs11970286CT基因型、TT+CT基因型可能是特发性室速发生的保护因素(OR=0.288,95%CI:0.138~0.599,P<0.001;OR=0.314,95%CI:0.163~0.606,P<0.001)。结论 rs11970286T等位基因及CT、TT+CT基因型是中国汉族人群特发性室速的保护因素,其作用机制可能与影响QT间期有关。
Objective To investigate the relationship between rs11970286 polymorphism and QT interval and ventricular tachycardia (VT) in Chinese Han population. Methods 242 patients with VT were ventricular tachycardia and 1002 healthy subjects were control group. ECG and heart rate, PR interval, QRS duration, QT interval and QTc interval were measured in both groups. Two sets of peripheral blood DNA were extracted and genotyped rs11970286 using Rotor-Gene TM6000 high resolution solution curve. Genomic software PLINK v1.05 was used to analyze the relationship between rs11970286 polymorphism and QT interval, VT, The occurrence of correlation analysis. Results Compared with the control group (5.49%, 0.80%) (P <0.05), the QT interval [(380.72 ± 58.82) ms] in the VT group was significantly higher than that in the control group [ (376.71 ± 27.92) ms]. There was a negative correlation between rs11970286T allele and QT interval in all subjects (β = -14.071, P = 0.018), but not with the adjusted QTc had no significant difference (P = -16.876, P = 0.062). There was no significant difference in the frequencies of rs11970286 genotype and allele between the two groups (P> 0.05) The frequencies of rs11970286CT genotype (16.981%) and TT + CT genotype (22.642%) in patients with idiopathic ventricular tachycardia were lower than those in control group (39.521%, 48.204%, P <0.05) (P> 0.05). The distribution frequency of TT allele (5.660%) was not significantly different from that of control group (8.682%) (P> 0.05), and the frequency of T allele was 14.151% lower than that of control group (28.443% 0.01). The T allele may be the protective factor of idiopathic VT (OR = 0.415, 95% CI: 1.385-4.1919, P <0.001). The rs11970286CT genotype and TT + CT genotype may be idiopathic Protection factor (OR = 0.288, 95% CI: 0.138-0.599, P <0.001; OR = 0.314, 95% CI: 0.163-0.606, P <0.001). Conclusion The rs11970286T allele and CT, TT + CT genotypes are the protective factors of idiopathic VT in Chinese Han population. Its mechanism may be related to the influence of QT interval.