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针对黄酮分子的酚羟基结构特点,有目的地选择了4种结构不同的商品化树脂,考察了它们对沙棘叶中黄酮类有效成分的吸附能力和吸附选择性.在此基础上优化了树脂结构设计方案,合成了具有氨基功能基、可形成氢键作用、疏水性可调变的大孔吸附树脂.以沙棘叶粗提物为原料,考察了树脂骨架疏水性、功能基间隔臂长短等对树脂纯化效果的影响规律.研究结果表明,当吸附发生在水体系中,一定强度的疏水性作用是树脂与吸附质之间形成氢键的必要条件,对于尺寸较大的黄酮分子,氢键功能基的间隔臂长短也显著影响了树脂对它的吸附能力.最后,我们选择了疏水性和氢键功能基间隔臂长短适宜的XM20-2树脂,对吸附和洗脱条件进行了优化,将其用于沙棘叶粗提物中黄酮类有效成分的进一步纯化,可将黄酮纯度从粗提物中的10.4%提高到50%以上,且树脂具有很好的重复使用性.
According to the structural characteristics of phenolic hydroxyl groups of flavone molecules, four commercially available resins with different structures were deliberately selected, and their adsorption capacity and adsorption selectivity on flavonoid active ingredients in seabuckthorn leaves were investigated. Based on this, the resin structure A macroporous adsorption resin with amino functional group, hydrogen bond formation and hydrophobic modification was synthesized.The crude extract of seabuckthorn was used as raw material to investigate the effects of resin backbone hydrophobicity and functional base length Resin purification effect.The results show that when the adsorption occurs in a water system, a certain degree of hydrophobic interaction is necessary for the formation of hydrogen bonds between the resin and the adsorbate. For the larger size of the flavone molecule, the hydrogen bonding function The length of the spacer on the base also significantly affected the adsorption capacity of the resin.Finally, we optimized the adsorption and elution conditions of XM20-2 resin with suitable hydrophobicity and hydrogen bond-based spacer length, For the further purification of active ingredients of flavonoids from crude extracts of seabuckthorn leaves, the purity of flavones can be increased from 10.4% of the crude extract to over 50%, and the resin has good repetition Sex.