论文部分内容阅读
制备胰高血糖素免疫敏感膜并建立一种快速、简便测定胰高血糖素含量的方法.利用化学自组装技术将胰高血糖素的抗体组装到SPR 基片上,首先通过对已知浓度的胰高血糖素进行SPR 检测,确定其结合常数,并以此对未知浓度的胰高血糖素进行了测定,样品无需纯化和标记.
Prepare glucagon immunosensitive membranes and establish a rapid and simple method for the determination of glucagon content. The use of chemical self-assembly technology to assemble glucagon antibody SPR substrate, first by known concentration of glucagon SPR test to determine the binding constant, and thus unknown concentration of glucagon carried out The assay did not require purification and labeling of samples.