论文部分内容阅读
目的对广州市医疗机构常用梅毒血清抗体检测试剂功效性进行评价。方法以美国BD公司快速血浆反应素环状卡片试验(RPR)检测试剂和日本富士瑞必欧株式会社的梅毒螺旋体颗粒凝集试验(TPPA)检测试剂作为参比试剂,评估4种非梅毒螺旋体血清抗体和5种梅毒螺旋体血清抗体检测试剂,每种试剂检测梅毒患者血清189例,健康对照30例,比较各试剂的敏感性、特异性和Kappa值。结果 4种非梅毒螺旋体血清抗体检测试剂和5种梅毒螺旋体血清抗体检测试剂与参比试剂相比,敏感性、特异性均大于95%,Kappa值均大于0.90,一致性好。结论广州市医疗机构选用试剂性能较好,试剂评估需每年坚持。
Objective To evaluate the efficacy of commonly used syphilis serum antibody detection reagents in medical institutions in Guangzhou. METHODS: Four non-Treponema pallidum serum antibody (anti-Treponema pallidum serogroup B) were tested using the Rapid Plasma Assay Cardin Receptor Card Reagent (RPR) from BD Company and Treponema pallidum Particle Agglutination Test (TPPA) from Japan Fu Shirayou Biotech Co., And five kinds of Treponema pallidum serum antibody test reagents, each reagent test syphilis patients serum 189 cases, 30 healthy controls, comparing the sensitivity, specificity and Kappa value of each reagent. Results The sensitivity and specificity of four kinds of non-Treponema pallidum serum antibody detection reagents and five kinds of Treponema pallidum serum antibody detection reagents were all greater than 95%, Kappa values were greater than 0.90, and the consistency was good. Conclusion Guangzhou medical institutions choose reagent performance is better, reagent assessment need to adhere to each year.