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目的:探讨桂皮酸对NB4细胞诱导分化的作用及其机制。方法:采用光镜观察形态变化,嗜银蛋白染色检测各组细胞AgNORs颗粒数,流式细胞术检测CD11b和CD33,免疫组化测定细胞c-myc、c-fos蛋白表达。结果:桂皮酸作用NB细胞后,CINN可诱导NB4细胞向终末细胞分化,分化率较对照组差异明显（P<0.01）;细胞AgNOR3颗粒数明显减少（P<0.01）;CD11b表达升高,CD33表达下降;c-myc表达下降,c-fos蛋白表达升高（P<0.05）。结论:桂皮酸能使NB4细胞分化成熟,增殖能力下降,其机制可能是通过凋控NB4细胞的增殖、分化相关的基因c-myc、c-fos表达来抑制细胞增殖,诱导细胞分化。
Objective: To investigate the effect of cinnamic acid on the differentiation of NB4 cells and its mechanism. Methods: Morphological changes were observed by light microscopy. The number of AgNORs particles in each group was detected by argyrophil staining. CD11b and CD33 were detected by flow cytometry. The expressions of c-myc and c-fos protein were detected by immunohistochemistry. Results: Cinnamic acid induced the differentiation of NB4 cells into terminal cells after cinnamic acid treatment (P <0.01). The number of AgNOR3 particles decreased significantly (P <0.01), the expression of CD11b increased, CD33 expression decreased; c-myc expression decreased, c-fos protein expression increased (P <0.05). Conclusion: Cinnamic acid can make NB4 cells differentiate and proliferate, which may be due to the inhibition of proliferation and differentiation of NB4 cells by regulating the expression of c-myc and c-fos genes related to the proliferation and differentiation of NB4 cells.