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目的探讨红细胞胞浆游离钙(RBC[Ca2+]i)水平在新生儿蛛网膜下腔出血(SAH)发病机制中的作用。方法选择2006年6月至2010年12月住院的38例SAH患儿作为研究对象,于生后2～3d、5～7d及10～14d后各采集静脉血一次,采用Fura-2/AM法检测RBC[Ca2+]i水平,并以同期出生的20例正常新生儿作为对照。结果①SAH患儿RBC[Ca2+]i水平显著高于对照组(P<0.01),RBC[Ca2+]i水平与SAH程度存在明显正相关(r=0.631,P<0.05)。②SAH患儿RBC[Ca2+]i水平于生后5～7d达到较高水平,然后逐渐恢复,至10～14d后仍较对照组增高(P<0.05)。结论 RBC[Ca2+]i参与了SAH的病理生理过程,在SAH发病机制中可能起重要作用,动态监测RBC[Ca2+]i水平可能有助于SAH的早期诊断和预后判断。
Objective To investigate the role of erythrocyte cytoplasmic free calcium (RBC [Ca2 +] i) in the pathogenesis of neonatal subarachnoid hemorrhage (SAH). Methods Thirty-eight children with SAH who were admitted to hospital from June 2006 to December 2010 were selected as study subjects. Venous blood was collected at 2 ~ 3d, 5 ~ 7d and 10 ~ 14d after birth. Fura-2 / AM The level of RBC [Ca2 +] i was detected and compared with 20 normal newborns born in the same period. Results ① The level of RBC [Ca2 +] i in children with SA was significantly higher than that in the control group (P <0.01). The level of RBC [Ca2 +] i was positively correlated with the level of SAH (r = 0.631, P <0.05). ② The level of RBC [Ca2 +] i in children with SAH was higher at 5 ~ 7d after birth and then recovered gradually, and still increased after 10 ~ 14d (P <0.05). Conclusions RBC [Ca2 +] i participates in the pathophysiological process of SAH and may play an important role in the pathogenesis of SAH. The dynamic monitoring of RBC [Ca2 +] i level may contribute to the early diagnosis and prognosis of SAH.