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新生儿(包括未成熟儿)败血症,至今仍然是儿科死亡率较高的一种常见病,由于其缺乏特异性症状,早期诊断困难。本文根据文献综述如下: 一、具有特异性的测定项目 (一)血浆棕黄层涂片检菌法:其方法是取末梢静脉血1ml,用肝素抗凝,离心后吸掉血浆上清液,取与红细胞相近的白细胞棕黄层作涂片,用美蓝及革兰染色各一张(因美蓝染色对革兰染色阴性细菌,尤其是大肠杆菌是很好的筛查染色法,美蓝染色对革兰阳性细菌效果好)进行镜检,若有一张血片中发现中性粒细胞内有2个以上细菌即为阳性。Brooks等报道此法诊断无1例假
Sepsis in newborns, including immature infants, is still a common disease with a high pediatric mortality rate and is difficult to diagnose early due to its lack of specific symptoms. This article based on the literature are summarized as follows: First, the specific determination of the project (a) plasma brown layer smear bacteria: The method is to take peripheral venous blood 1ml anticoagulant with heparin, centrifuged after aspiration of plasma supernatant, Take white blood cells similar to the red blood cells brown layer smear, with the United States Blue and Gram stain each one (due to methylene blue staining of gram-negative bacteria, especially E. coli is a good screening staining method, Staining of Gram-positive bacteria effect is good) microscopic examination, if a blood film found in more than 2 neutrophils bacteria is positive. Brooks et al reported that there was no one case of this diagnosis