槟榔碱和烟碱对离体大鼠海马脑片CA_1区锥体细胞诱发电位的影响

• 作 者: 杨爱珍 刘传绩
• 机 构: 军事医学科学院在物药物研究所,军事医学科学院在物药物研究所 北京 100850,北京 100850
• 出 处: 中国应用生理学杂志
• 发表日期: 1993年01期
• 关键词: 海马脑片 锥体细胞 烟碱 CA_1 槟榔碱 群峰电位 受体激动剂 细胞外记录 离体大鼠 浴槽

在离体海马脑片上,用微电极细胞外记录刺激底树突层Schaff纤维引起的CA_1区锥体细胞顺向群峰电位(PS)。观察在浴槽中给予M受体激动剂槟榔碱(Are)和N受体激动剂烟碱(Nic)对PS的影响,探讨海马M和N受体功能及它们之间的关系。 Are 1μmol/L(即阈浓度)开始能使PS幅度下降为正常的74％,随浓度增大,PS下降更明显,当浓度增加至1mmol/L时,PS下降为正常的63％,有一定量效关系,但斜率很小。对PS波形、时程及潜伏期均无影响。用人

In isolated rat hippocampal slices, the forward peak potentials (PSs) of CA 1 pyramidal cells were stimulated by microelectrode extracellular recording with Schaff fibers. The effect of M receptor agonist Are and N receptor agonist nicotine on PS in the bath was observed to investigate the function of M and N receptors in hippocampus and the relationship between them. When the concentration of 1 μmol / L (ie, the threshold concentration) began to decrease the amplitude of PS to 74% of normal, the PS decreased more obviously with increasing concentration. When the concentration increased to 1 mmol / L, the PS decreased to 63% Effective relationship, but the slope is small. No effect on PS waveform, duration and latency. Employ people