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以水稻簇矮病株或带毒昆虫的榨出液,注射于Nephotettix cincticeps体内,依其日后对稻苗的侵染力,测得水稻簇矮病毒(RBSV)的致死温度为60～70℃,稀释限点为10~(-4)～10~(-5)(病叶榨出液)或10~(-3)～10~(-4)(带毒虫榨出液),体外存活期为4～5天(在4℃下)或2～3天(在22℃下)。RBSV在病株中以叶片汁液的侵染力最强(感染率为25.0%),叶鞘次之(10.5%),根系最低(4.8%),茎秆未能测出。病毒注射虫体后的循回期在26.9℃下为7～17天,平均10.3天,比常规饲毒的要短。病毒在虫体内自饲毒后需10天以上才能测出侵染力,但以25天的最强。病害的潜育期(在27℃下)为8～22天,一般多在13～14天。病毒接种后在稻株体内10天即具侵染力,但以30天的最强。
Nephrotoxicity was induced in Nephotettix cincticeps by measuring the infestation of rice cluster dwarf virus and insect-infected dwarf virus, and the lethal temperature of rice cluster dwarf virus (RBSV) was 60-70 ℃. The limit of dilution was 10 ~ (-4) ~ 10 ~ (-5) (diseased leaf extract) or 10 ~ (-3) ~ 10 ~ (-4) 4 to 5 days at 4 ° C or 2 to 3 days at 22 ° C. In the diseased plants, RBSV had the strongest infectivity in leaf juice (infection rate was 25.0%), leaf sheath (10.5%), and root system lowest (4.8%). The recovery period after virus injection was 7 to 17 days at 26.9 ℃, an average of 10.3 days, which was shorter than that of routine feeding. Viruses in the parasites need more than 10 days after infecting themselves to detect infectivity, but the strongest in 25 days. The incubation period of disease (at 27 ℃) is 8 to 22 days, usually more than 13 to 14 days. Inoculation of the virus in rice plants within 10 days that is invasive, but the strongest in 30 days.