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目的 探讨胃癌组织、癌旁组织及胃溃疡组织中转化生长因子 α(TGF α)mRNA表达水平变化及其临床意义。方法应用逆转录—聚合酶链反应法 (RT PCR )和数字成像扫描技术 ,对 2 3例胃癌组织、癌旁组织及 11例胃溃疡组织中TGF αmRNA进行定量检测。结果 胃癌组织及癌旁正常组织中TGF αmRNA表达水平均明显高于胃溃疡组织 (P <0 .0 5 ) ;胃癌组织与癌旁组织中TGF αmRNA表达无显著性差异 ;胃癌TGF αmRNA表达与肿瘤分期、淋巴结转移无关。结论 TGF αmRNA过度表达与胃癌发生呈正相关作用 ,是胃癌前病变的 1项有意义的指标 ,有助于胃癌患者的早期发现。
Objective To investigate the changes of mRNA expression of transforming growth factor-α (TGF-α) and its clinical significance in gastric cancer tissues, adjacent non-cancerous tissues and gastric ulcer tissues. Methods Reverse transcription polymerase chain reaction (RT PCR) and digital imaging scanning technique were used to quantitatively detect TGF α mRNA in 23 gastric cancer tissues, adjacent non-cancerous tissues and 11 gastric ulcer tissues. Results The expression levels of TGF α mRNA in gastric cancer tissues and adjacent normal tissues were significantly higher than those in gastric ulcer tissues (P <0.05). There was no significant difference in the expression of TGF α mRNA between gastric cancer tissues and adjacent normal tissues. Staging, lymph node metastasis has nothing to do. Conclusion The overexpression of TGF α mRNA is positively correlated with the occurrence of gastric cancer, which is a meaningful indicator of gastric precancerous lesions and contributes to the early detection of gastric cancer.