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[目的]对比观察得斯芬与扶正解毒抗癌复方(中药复方)对Bel-7402细胞增殖和凋亡的影响。[方法]2种药物分别作用Bel-7402细胞48 h,以EnVision二步法检测细胞Bcl-2、P53蛋白的表达;以流式细胞仪进行凋亡率和细胞周期分析。[结果]50、25μmol/L得斯芬组与102、mg/ml中药复方组P53表达与对照组比较差异有统计学意义(P<0.05或<0.01);50、25μmol/L得斯芬组与10 mg/ml中药复方组在G1峰前出现了明显凋亡峰(Ap峰),凋亡率分别为23.9%、14.7%、5.5%。[结论]得斯芬与扶正解毒抗癌复方可能通过上调P53蛋白的表达、抑制肿瘤细胞DNA合成,使细胞周期停滞在G0/G1期,阻滞细胞进入G2/M期,导致肝癌细胞凋亡。比较结果,得斯芬的抗肝癌效应似乎比扶正解毒抗癌复方更为优越。
[Objective] To compare and observe the effects of Defensin and Fuzheng Jiedu anticancer compound (traditional Chinese medicine compound) on the proliferation and apoptosis of Bel-7402 cells. [Methods] Bel-7402 cells were treated by two drugs for 48 h, respectively. The expression of Bcl-2 and P53 protein was detected by EnVision two-step method. The apoptosis rate and cell cycle were analyzed by flow cytometry. [Results] The expression of P53 in 50, 25μmol / L dasatingen group and 102, mg / ml traditional Chinese medicine compound group had significant difference compared with the control group (P <0.05 or <0.01); 50, 25μmol / Compared with 10 mg / ml traditional Chinese medicine compound group, obvious peak of apoptosis (Ap peak) appeared before G1 peak, the apoptosis rates were 23.9%, 14.7% and 5.5% respectively. [Conclusion] Defensin and Fuzheng Jiedu anticancer compound may inhibit the DNA synthesis of tumor cells by up-regulating the expression of P53 protein, arrest the cell cycle in G0 / G1 phase and arrest the cells into G2 / M phase, leading to the apoptosis of hepatoma cells . The results showed that the anti-hepatoma effect of dermatan appeared to be superior to that of the compound prescription of detoxification and anti-cancer.