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蓝藻是古老的原核光合生物,不仅在植物进化上有重要地位,也是可利用的生物资源及重要的基因资源。Slr1515是来源于集胞藻6803(Synechocystis sp.PCC 6803)的基因,其同源基因在蓝藻中普遍存在,但对其生物学功能所知有限。以易于转化的水稻品种中花11(Oryza sativa subsp.japonica cv.‘Zhonghua 11’)为材料,通过农杆菌介导的遗传转化方法将Slr1515基因导入水稻胚性愈伤组织中。经过诱导分化,获得了一系列转基因幼苗。通过PCR、GFP荧光及免疫印迹检测,筛选出单拷贝插入的转基因阳性株系,并对野生型和转基因株系的表型进行比较分析。结果表明,灌浆期转基因株系的剑叶长度比同期的野生型提高了27.7%。进一步的测定结果显示,转基因株系的剑叶叶绿素含量和PSII最大量子产率分别比野生型提高了0.65倍和4.4%。推测Slr1515基因可能在改善水稻农艺性状方面有一定价值。
Cyanobacteria are ancient prokaryotic photosynthetic organisms that not only play an important role in the evolution of plants, but also are the available biological resources and important genetic resources. Slr1515 is a gene derived from Synechocystis sp. PCC 6803 whose homologous genes are ubiquitous in cyanobacteria but have limited knowledge of their biological functions. The Oryza sativa subsp. Japonica cv. ’Zhonghua 11’ was transformed into rice embryogenic callus by Agrobacterium tumefaciens-mediated genetic transformation. After induction of differentiation, a series of transgenic seedlings were obtained. By PCR, GFP fluorescence and Western blot detection, single copy inserted transgenic positive lines were screened and the phenotypes of wild-type and transgenic lines were compared. The results showed that the flag leaf length of transgenic lines at grain filling stage increased by 27.7% compared with the wild type at the same period. Further measurements showed that the flag leaf chlorophyll content and PSII maximum quantum yield of the transgenic lines increased by 0.65 times and 4.4% respectively than the wild type. Speculated Slr1515 gene may have some value in improving the agronomic traits of rice.