论文部分内容阅读
AIM:To investigate the chemo preventive effects ofvanadium on rat colorectal carcinogenesis induced by 1,2-dimethylhydrazine (DMH).METHODS:Male Sprague-Dawley Rats were randomlydivided into four groups.Rats in Group A received salinevehicle alone for 16 weeks.Rats in Group B were givenDMH injection once a week intraperitoneally for 16 weeks;rats in Group C,with the same DMH treatment as in theGroup B,but received 0.5-ppm vanadium in the formammonium monovanadate ad libitum in drinking water.Ratsin the Group D received vanadium alone as in the Group Cwithout DMH injection.RESULTS:Aberrant crypt foci (ACF) were formed inanimals in DMH-treated groups at the end of week 16.Compared to DMH group,vanadium treated group had lessACF (P<0.001).At the end of week 32,all rats in DMHgroup developed large intestinal tumors.Rats treated withvanadium contained significantly few colonic adenomas andcarcinomas (P<0.05) compared to rats administered DMHonly.In addition,a significant reduction (P<0.05) in colontumor burden (sum of tumor sizes per animal) was alsoevident in animals of Group C when compared to those inrats of carcinogen control Group B.The results also showedthat vanadium significantly lowered PCNA index in ACF(P<0.005).Furthermore,vanadium supplementation alsoelevated liver GST and Cyt P-450 activities (P<0.001 andP<0.02,respectively).CONCLUSION:Vanadium in the form of ammoniummonovanadate supplemented in drinking water ad libitumhas been found to be highly effective in reducing tumorincidence and preneoplastic foci on DMH-inducedcolorectal carcinogenesis.These findings suggest thatvanadium administration can suppress colon carcinogenesisin rats.
AIM: To investigate the chemo preventive effects ofvanadium on rat colorectal carcinogenesis induced by 1,2-dimethylhydrazine (DMH) .METHODS: Male Sprague-Dawley Rats weredivided into four groups. Rats in Group A received salinevehicle alone for 16 weeks.Rats in Group B were given an injection of one-week intraperitoneally for 16 weeks; rats in Group C, with the same DMH treatment as in the Group B, but received 0.5-ppm vanadium in the formammonium monovanadate ad libitum in drinking water. Ratsin the Group D received vanadium alone as in the Group Cwithout DMH injection. RESULTS: Aberrant crypt foci (ACF) were formed in animals in DMH-treated groups at the end of week 16. Compared to DMH group, vanadium treated group had less ACF (P <0.001) .At the end of week 32, all rats in DMHgroup developed large intestinal tumors. Rats treated with vanadium containing significantly fewer colonic adenomas and carcinomas (P <0.05) compared to rats administered DM Hunly.In addition, a significant reduction (P <0.05) i n colontumor burden (sum of tumor sizes per animal) was alsoevident in animals of Group C when compared to those inrats of carcinogen control Group B.The results also showed that the PCNA index in ACF (P <0.005) .Furthermore, vanadium supplementation also colevated liver GST and Cyt P-450 activities (P <0.001 and P <0.02, respectively) .CONCLUSION: Vanadium in the form of ammoniummonovanadate supplemented in drinking water ad libitumhas been found to be highly effective in reducing tumorincidence and preneoplastic foci on DMH-inducedcolorectal carcinogenesis.These findings suggest thatvanadium administration can suppress colon carcinogenesis in rats.