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目的探讨全氟辛烷磺酸(Perfluorooctane,sulfonate,PFOS)经口亚慢性染毒致大鼠肝脏氧人损伤及对脂褐质(Lipofuscin,LF)含量的影响。方法24只雄性SD大鼠按体重随机分为4组,各染毒组饮水中PFOS浓度分别为1.7,5.0,15.0 mg/L。连续染毒90 d后,观察大鼠肝脏脏器系数;测定大鼠血清PFOS浓度、肝脏丙二醛(MDA)、LF含量和总抗氧化能力(T-AOC)。结果各染毒组与对照组大鼠体重差异无统计学意义(P>0.05);各染毒组血清PFOS浓度均显著高于对照值(P<0.05);5.0和15.0 mg/L染毒大鼠肝脏重量分别为(19.5±1.3),(20.4±4.8)g,显著高于对照值(14.2±3.4)g(P<0.05)。肝脏脏器系数分别为(4.20±0.18)%,(4.95±0.56)%,显著高于对照值(3.54±0.41)%(P<0.05)。肝脏MDA含量分别(2.38±0.92),(1.82±0.48)nmol/(mg.prot),显著高于对照值(0.87±0.11)nmol/(mg.prot)(P<0.05)。T-AOC分别为(1.33±0.20),(1.35±0.18)U/(mg.prot),显著高于对照值(1.08±0.17)U/(mg.prot)(P<0.05);15.0 mg/L染毒组大鼠肝脏LF含量为(2.89±0.15)μg/ml,显著高于对照值(2.68±0.09)μg/ml(P<0.05)。结论PFOS可致大鼠肝脏氧化损伤并增加肝脏脂褐质含量。
Objective To investigate the effects of perfluorooctane sulfonate (PFOS) on liver oxygenation and lipofuscin (LF) in rats. Methods Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups according to body weight. The concentrations of PFOS in drinking water of each exposure group were 1.7, 5.0 and 15.0 mg / L, respectively. After continuous exposure for 90 days, the indexes of liver organ in rats were observed. The levels of serum PFOS, liver MDA, LF and T-AOC were measured. Results There was no significant difference in the weight of rats in each exposure group and the control group (P> 0.05). The concentrations of PFOS in each exposure group were significantly higher than those in the control group (P <0.05). The exposure rates of 5.0 and 15.0 mg / L The liver weights of rats were (19.5 ± 1.3) and (20.4 ± 4.8) g, respectively, which were significantly higher than those of the control group (14.2 ± 3.4) g (P <0.05). The coefficients of liver organ were (4.20 ± 0.18)% and (4.95 ± 0.56)%, respectively, which were significantly higher than those of the control (3.54 ± 0.41)% (P <0.05). The liver MDA content was (2.38 ± 0.92) and (1.82 ± 0.48) nmol / (mg.prot) respectively, which was significantly higher than that of the control (0.87 ± 0.11) nmol / (mg.prot) (P <0.05). T-AOC was (1.33 ± 0.20) and (1.35 ± 0.18) U / (mg.prot) respectively, which was significantly higher than the control (1.08 ± 0.17) U / (mg.prot) LF content in liver of L group was (2.89 ± 0.15) μg / ml, which was significantly higher than that of control group (2.68 ± 0.09) μg / ml (P <0.05). Conclusion PFOS can cause oxidative damage in rat liver and increase hepatic lipofuscin content.