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利用生物信息学方法,从辣椒全基因组数据库中共鉴定出11个辣椒TPS基因,除Ca TPS3含有1个TPS和2个TPP结构域外,其余分别含有1个TPS和1个TPP结构域。11个Ca TPS分为2类,ClassⅠ包含3个基因(Ca TPS1~Ca TPS3),分别含有16、16和13个内含子,Ca TPS1和Ca TPS2含Motif 1~Motif 5各1个,而Ca TPS3含有2个Motif 4和1个Motif 3。ClassⅡ包含8个基因(Ca TPS4~Ca TPS11),均含有2个内含子,且分别含Motif 1~Motif 6各1个。利用荧光定量PCR对Ca TPS1在辣椒组织表达的特异性和低温应答模式进行分析,结果表明:Ca TPS1在叶片中的表达量大约是根和茎中的5~6倍,说明Ca TPS1的表达具有明显的组织特异性;低温处理后Ca TPS1在根和茎中的表达高峰出现时间明显早于叶片,说明不同组织中Ca TPS1对低温胁迫的应答时间存在一定差异。
Using bioinformatics method, 11 capsicum TPS genes were identified from pepper genome database, except for one TPS and two TPP domains of Ca TPS3, and one TPS and one TPP domain respectively. Eleven Ca TPSs were divided into two groups, Class I contained three genes (Ca TPS1-Ca TPS3) containing 16, 16 and 13 introns respectively, Ca TPS1 and Ca TPS2 each containing one Motif 1 to Motif 5, and Ca TPS3 contains 2 Motif 4 and 1 Motif 3. Class II contains eight genes (Ca TPS4 ~ Ca TPS11), each containing two introns, each containing one Motif 1 ~ Motif 6 each. The results showed that the expression level of Ca TPS1 in leaves was about 5-6 times higher than that in roots and stems, indicating that the expression of Ca TPS1 has Obvious tissue specificity. The expression peak of Ca TPS1 in roots and stems was earlier than that of leaves at low temperature, indicating that Ca TPS1 had different response time to low temperature stress in different tissues.