论文部分内容阅读
目的研究IL-13对人肺成纤维细胞株HFL-1和人肝星状细胞株LX-2 IL-13受体和IL-4受体表达的调节作用。方法 RT-PCR法检测HFL-1细胞株和LX-2细胞株IL-13Rα1、IL-4R和IL-13Rα2 mRNA的表达;凝胶电泳定量软件Image Tool2.0对RT-PCR电泳条带进行光密度分析;ELISA法检测HFL-1细胞株和LX-2细胞株分泌可溶型IL-13Rα2以及检测细胞裂解液总IL-13Rα1、IL-4R和IL-13Rα2含量。结果 IL-13(5～100 ng/ml)对HFL-1细胞株和LX-2细胞株表达IL-13Rα1和IL-4R无影响;IL-13为5、10、20 ng/ml时能诱导HFL-1细胞株表达IL-13Rα2并呈现剂量依赖,当IL-13为50 ng/ml时,对HFL-1细胞株IL-13Rα2表达的诱导作用明显减弱,IL-13 100 ng/ml组没有检测到HFL-1细胞株IL-13Rα2的表达;LX-2细胞株IL-13Rα2表达缺失且IL-13不能诱导LX-2细胞株表达IL-13Rα2。结论 IL-13不能上调人肺成纤维细胞株HFL-1和人肝星状细胞株LX-2表达功能型受体IL-13Rα1和IL-4R,表明IL-13不能通过上调IL-13Rα1和IL-4R表达量来放大自身作用;一定浓度的IL-13能诱导人肺成纤维细胞株HFL-1表达抑制型受体IL-13Rα2,表明IL-13的自身负调控。
Objective To investigate the regulatory effect of IL-13 on the expression of IL-13 receptor and IL-4 receptor in human lung fibroblast cell line HFL-1 and human hepatic stellate cell line LX-2. The expression of IL-13Rα1, IL-4R and IL-13Rα2 mRNA in HFL-1 cell line and LX-2 cell line was detected by RT-PCR. The RT-PCR electrophoresis band The soluble IL-13Rα2 secreted by HFL-1 cell line and LX-2 cell line was detected by ELISA, and the total IL-13Rα1, IL-4R and IL-13Rα2 contents in cell lysate were measured. Results IL-13 (5-100 ng / ml) had no effect on the expression of IL-13Rα1 and IL-4R in HFL-1 cells and LX-2 cells. When IL-13 was 5, 10 and 20 ng / ml, IL-13Rα2 was expressed in a dose-dependent manner in HFL-1 cells. When IL-13 was 50 ng / ml, the induction of IL-13Rα2 expression in HFL-1 cells was significantly attenuated The expression of IL-13Rα2 in HFL-1 cell line was detected. The expression of IL-13Rα2 in LX-2 cell line was absent and IL-13 could not induce the expression of IL-13Rα2 in LX-2 cell line. Conclusion IL-13 can not up-regulate the expression of IL-13Rα1 and IL-4R in human lung fibroblast cell line HFL-1 and human hepatic stellate cell line LX-2, indicating that IL-13 can not up-regulate IL-13Rα1 and IL- -4R expression to amplify its own role; a certain concentration of IL-13 can induce human lung fibroblast cell line HFL-1 expression of inhibitory receptor IL-13Rα2, indicating that IL-13 self-negative regulation.