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目的探讨微管调节蛋白Stathmin对卵巢癌C13K细胞侵袭和迁移性的影响及机制研究。方法选择C13K细胞为实验对象,应用siRNA靶向沉默Stathmin,利用实时定量PCR(qRT-PCR)方法检测转染48 h后转染效果,transwell法和细胞划痕实验测定细胞侵袭和迁移能力变化,qRT-PCR检测侵袭相关基因MMP2和MMP9的变化。结果与空白对照组和阴性对照组相比较,Stathmin siRNA明显降低了C13K细胞中Stathmin mRNA的表达量,StathminsiRNA组侵袭的细胞数和细胞损伤愈合的速度明显降低,Stathmin-siRNA组MMP2和MMP9表达量明显降低。结论沉默Stathmin表达能有效抑制卵巢癌细胞的侵袭迁移能力,其机制可能与抑制MMP2和MMP9的表达有关,为卵巢癌的治疗前景增加新的希望。
Objective To investigate the effect of microtubule regulatory protein Stathmin on the invasion and migration of ovarian cancer C13K cells and its mechanism. Methods C13K cells were selected as experimental subjects. SiRNA targeting silencing Stathmin was used to detect the transfection efficiency by real-time quantitative PCR (qRT-PCR) 48 h after transfection. Transwell assay and cell scratch assay were used to determine the changes of cell invasion and migration. qRT-PCR detection of invasion-related genes MMP2 and MMP9 changes. Results Compared with the blank control group and the negative control group, Stathmin siRNA significantly reduced the expression of Stathmin mRNA in C13K cells. The number of invasion and metastasis of StathminsiRNA group decreased significantly. The expression of MMP2 and MMP9 in Stathmin-siRNA group Obvious reduction. Conclusions Silencing Stathmin expression can effectively inhibit the invasion and migration of ovarian cancer cells. The mechanism may be related to the inhibition of the expression of MMP2 and MMP9, so as to add new hope for the treatment of ovarian cancer.