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目的：观察大鼠脑缺血再灌流损伤后脑源性神经营养因子（ＢＤＮＦ）和睫状神经营养因子（ＣＮＴＦ）ｍＲＮＡ在海马中的分布及其表达。方法：大鼠颈总动脉夹闭造成脑缺血３０ｍｉｎ，于６，１２，２４，７２ｈ进行点杂交和原位杂交，７２ｈ时进行神经元尼氏小体染色。结果：脑缺血后，海马ＣＡ１区神经元大量死亡，ＢＤＮＦｍＲＮＡ表达于６ｈ开始增加，７２ｈ恢复正常。２４ｈ时齿状回ＢＤＮＦｍＲＮＡ表达高于ＣＡ１区。ＣＮＴＦｍＲＮＡ表达于１２ｈ开始持续增加。结论：脑缺血再灌流损伤后，海马ＢＤＮＦｍＲ－ＮＡ和ＣＮＴＦｍＲＮＡ的表达均增加。ＢＤＮＦ可能有利于齿状回神经元耐受缺血，ＣＮＴＦ可能在局部发挥神经营养作用
AIM: To observe the distribution and expression of brain derived neurotrophic factor (BDNF) and ciliary neurotrophic factor (CNTF) mRNA in hippocampus after cerebral ischemia-reperfusion injury in rats. Methods: The rats were subjected to carotid artery occlusion for 30 min after cerebral ischemia. Dot hybridization and in situ hybridization were performed at 6, 12, 24 and 72 h, and neuronal Nissl body staining was performed at 72 h. Results: After cerebral ischemia, neurons in CA1 area of hippocampus died in a large number. BDNF mRNA expression increased at 6h and returned to normal at 72h. The expression of BDNF mRNA in dentate gyrus at 24h was higher than that in CA1. CNTF mRNA expression continued to increase from 12h. Conclusion: After cerebral ischemia-reperfusion injury, the expression of BDNFmR-NA and CNTF mRNA in the hippocampus increased. BDNF may favor dentate neuronal tolerance to ischemia, and CNTF may exert neurotrophic effects locally