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以‘晋荞2号’、‘迪苦1号’、‘西农9909’、‘西农9920’、‘西农9940’、‘西农9943’、‘黔苦2号’、‘黔苦3号’、‘黔苦4号’、‘黔苦5号’、‘黔苦7号’、‘昭苦1号’、‘‘昭苦2号’、‘‘西荞2号’、‘西荞3号’、‘云荞1号’、‘云荞2号’、‘九江苦荞’和‘榆6-21’等19个苦荞品种为实验材料,NaCl胁迫下测定相关耐盐生理指标,并对耐盐基因FtNHX1表达量进行分析,以筛选耐盐和盐敏感苦荞品种。结果表明,NaCl胁迫下‘黔苦3号’种子发芽率和幼苗SOD活性降低幅度较小,幼苗质膜透性和MDA含量增加幅度较小,说明盐胁迫下‘黔苦3号’生理特性较为稳定,且耐盐基因FtNHX1在主要拒Na+部位表达量的增加幅度最大,可以有效减少盐分向地上部运输;而‘西农9909’幼苗质膜透性和MDA含量增加幅度较大,说明盐胁迫下其膜系统受到很大伤害,且幼苗叶绿素含量和SOD活性降低幅度较大。因此,19个苦荞品种中‘黔苦3号’的耐盐性最强,是耐盐品种;‘西农9909’的耐盐性最差,是盐敏感品种。
The production of ’Jinqiao 2’, ’Di Bit 1’, ’Ximeng 9909’, ’Ximeng 9920’, ’Ximeng 9940’, ’Ximeng 9943’, ’Guizhou Bitter 2’ ’Qiaochang 4’, ’Guizhou bitter 5’, ’Guizhou bitter 7’, ’Zhaobei No. 1’, ’’ Zhao Zhaoyu 2 ’,’ ’West buckwheat 2’, ’West buckwheat’ 19 buckwheat cultivars such as ’No.3’, ’Yunghuankai 1’, ’Yunghuankou 2’, ’Jiujiang Tartary Buckwheat’ and ’Yu 6-21’ were used as experimental materials. The physiological indexes of salt tolerance were determined under NaCl stress, The expression level of FtNHX1, a salt tolerant gene, was analyzed to screen salt-tolerant and salt-sensitive buckwheat varieties. The results showed that the seed germination rate and seedling SOD activity of ’Guizhou bitter 3’ decreased less under NaCl stress, and the increase of plasma membrane permeability and MDA content in seedling was less, which indicated that the physiological characteristics of ’Guizhou bitter 3’ Stable, and salt-tolerant gene FtNHX1 mainly rejects the Na + part of the expression of the maximum increase, which can effectively reduce the salinity to the upper part of the transport; and ’Westong 9909’ seedling membrane permeability and MDA content increased more, indicating that salt stress Under the membrane system was greatly damaged, and the seedling chlorophyll content and SOD activity decreased significantly. Therefore, 19 varieties of bitter buckwheat ’Guizhou bitter 3’ the most salt-tolerant, salt-tolerant varieties; ’Xiunong 9909’ the worst salt tolerance is salt-sensitive varieties.