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应用聚合酶链反应（ＰＣＲ）检测急性和慢性乙肝患者外周血单个核细胞（ＰＢＭＣ）中的ＨＢＶ－ＤＮＡ，阳性率为６３．８％。其中，急肝患者阳性率为７８．６％；慢迁肝患者为５６．３％；慢活肝患者为５７．１％。ＰＢＭＣ中ＨＢＶ－ＤＮＡ与血清ＨＢＶ－ＤＮＡ和ｅ抗原有一致性，但ＨＢｅＡｇ阴性者中也有部分患者的ＰＢＭＣ中有ＨＢＶ－ＤＮＡ存在。本法消除了血清中ＨＢＶ－ＤＮＡ和ＰＢＭＣ自身ＤＮＡ的干扰，并且与传统分子杂交方法相比，工作量大大减少。
The positive rate of HBV-DNA in peripheral blood mononuclear cells (PBMC) of patients with acute and chronic hepatitis B was 63.8% by polymerase chain reaction (PCR). Among them, the positive rate of acute liver disease was 78.6%; slow-moving liver was 56.3%; slow-living liver was 57.1%. PBMC HBV-DNA and serum HBV-DNA and e antigen consistency, but there are some patients with HBeAg-negative PBMC also exist HBV-DNA. This method eliminates the interference of serum HBV-DNA and PBMC’s own DNA, and greatly reduces the workload compared with the traditional molecular hybridization method.