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现已发现的玉米小斑病Ｃ小种５２３菌株对玉米Ｃ型雄性不育细胞质材料的致病性比正常胞质材料严重．为了研究这种差异产生的可能机制，利用０．３％小斑病毒素处理正常胞质（Ｎ）和Ｃ细胞质材料线粒体，然后令线粒体进行体外翻译，发现离体线粒体蛋白质的合成都未受到影响，表明毒素没有改变Ｎ，Ｃ胞质线粒体膜的通透性，使氧化－磷酸化解偶联，也未直接抑制线粒体蛋白质的合成．将玉米叶片浸泡于０．３％的毒素溶液中处理，在不同处理时间测定溶液电导率，发现玉米叶片细胞膜通透性很快发生变化．用 ０．０５％毒素处理叶片后测定溶液电导率，发现Ｃ胞质材料叶片细胞对毒素较Ｎ和ＥＳ敏感．结果表明，毒素的作用位点在细胞质膜上而不在线粒体膜上，Ｃ胞质材料和Ｎ胞质材料感病严重性的差别是由细胞膜的稳定性导致的，而不是由不同胞质线粒体膜结构的差异导致的．
It has been found that the maize small spot disease C race 523 strains of maize C-type cytoplasmic male sterile material than the normal cytoplasmic material serious. To investigate the possible mechanism by which this difference occurs, 0.3% PBX was used to treat normal cytoplasmic (N) and C-cytoplasmic material mitochondria, which were then translated in vitro and found that the synthesis of isolated mitochondrial proteins was unaffected , Indicating that toxins did not change the permeability of N, C cytoplasmic mitochondrial membranes, uncoupled the oxidation-phosphorylation, nor did they directly inhibit mitochondrial protein synthesis. The maize leaves were immersed in 0.3% toxin solution for treatment. The conductivity of the solution was measured at different treatment times and the membrane permeability of maize leaves was rapidly changed. After the leaves were treated with 0.05% toxin, the conductivity of the solution was measured and it was found that leaf cells of C cytoplasm were sensitive to toxins compared to N and ES. The results showed that the toxin sites on the plasma membrane rather than the mitochondrial membrane C cytoplasmic material and N cytopathic material susceptibility to the severity of the difference caused by the stability of the cell membrane rather than by the different cytoplasmic mitochondrial membrane Structural differences result.