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目的:观察SHIP在溃疡性结肠炎(UC)和克罗恩病(CD)患者结肠组织标本中的表达情况,探讨其在炎症性肠病发生过程中所起的作用及意义。方法:收集活动期UC患者,活动期CD患者,及结直肠癌旁正常粘膜组织(NC组)标本各20例。将活检标本进行苏木精-伊红染色及SHIP免疫组化染色观察;利用Western blot半定量比较分析SHIP蛋白表达及组间差异;Real-time RT-PCR分析SHIP在RNA水平的表达情况和组间差异。统计学处理采用Student’s t检验。结果:免疫组化染色示UC组SHIP阳性表达积分为(7.20±2.53),CD组积分为(6.50±2.76),对照组积分为(1.10±0.74)。t检验组间比较UC组和CD组无统计学差异(t=0.59,P>0.05);而UC组与NC组(t=7.32,P<0.05),CD组与NC组(t=5.98,P<0.05),差异均有统计学意义。Western blot检测结肠组织SHIP表达,UC组SHIP相对表达量为(0.314±0.021),CD组(0.301±0.019),NC组(0.163±0.027)。UC和CD组表达无差异(t=1.44,P>0.05),而UC组,CD组与NC组相比表达明显升高(t=13.88、13.16,P均<0.05)。Real-time RT PCR检测UC组结肠粘膜SHIP m RNA相对表达量为(0.649±0.028),CD组为(0.645±0.021),NC组为(0.140±0.015)。同样,UC组与CD组没有统计学差异,而其相较对照组表达均升高(P<0.05)。结论:炎症性肠病患者结肠组织SHIP表达明显高于正常结肠组织,但其在溃疡性结肠炎和克罗恩病间没有明显差异;提示SHIP可能在炎症性肠病的发病中发挥重要作用。
Objective: To investigate the expression of SHIP in colonic mucosa of patients with ulcerative colitis (UC) and Crohn ’s disease (CD) and to explore the role and significance of SHIP in the pathogenesis of inflammatory bowel disease. Methods: Twenty patients with active UC, active CD and normal colorectal mucosa (NC) were collected. Biopsy specimens were harvested for hematoxylin-eosin staining and SHIP immunohistochemical staining. Semi-quantitative Western blot analysis was used to analyze SHIP protein expression and differences between groups. Real-time RT-PCR was used to analyze SHIP expression at RNA level and group Difference between. Statistical analysis using Student’s t test. Results: Immunohistochemical staining showed that the positive expression score of SHIP in UC group was (7.20 ± 2.53), the score of CD group was (6.50 ± 2.76), and the score of control group was (1.10 ± 0.74). (t = 0.59, P> 0.05). There was no significant difference between the UC and NC groups (t = 7.32, P <0.05) P <0.05), the differences were statistically significant. The expression of SHIP in colonic tissue was detected by Western blot. The relative expression of SHIP in UC group was (0.314 ± 0.021), CD group (0.301 ± 0.019), and NC group (0.163 ± 0.027). There was no significant difference between UC and CD groups (t = 1.44, P> 0.05). The expression of UC and CD groups was significantly higher than NC group (t = 13.88 and 13.16, P <0.05). Real-time RT-PCR showed that the relative expression of SHIP m RNA in colonic mucosa of UC group was (0.649 ± 0.028), (0.645 ± 0.021) in CD group and (0.140 ± 0.015) in NC group. Similarly, there was no significant difference between UC group and CD group (P <0.05). Conclusion: The expression of SHIP in colonic tissue of patients with inflammatory bowel disease was significantly higher than that of normal colonic tissue, but there was no significant difference between ulcerative colitis and Crohn ’s disease. It suggests that SHIP may play an important role in the pathogenesis of inflammatory bowel disease.