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目的:建立同时测定金银花中绿原酸、芦丁和木犀草苷含量的方法。方法:应用高效液相色谱法测定,色谱柱为Agilent Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为1.0 mL.min-1,柱温30℃;检测波长355 nm。结果:绿原酸、芦丁和木犀草苷的线性范围分别为12.21～203.5μg.mL-1(r=0.9992),2.653～132.6μg.mL-1(r=0.9999),2.794～139.7μg.mL-1(r=0.9995);平均加样回收率分别为98.77%(RSD=1.69%),99.52%(RSD=1.01%),100.84%(RSD=1.59%)。结论:该方法同时测定金银花中的绿原酸、芦丁、槲皮素和木犀草苷的含量,简便易行、准确、重复性好,可为金银花药材的质量控制研究提供一种可靠的参考方法。
Objective: To establish a method for simultaneous determination of chlorogenic acid, rutin and luteolin in honeysuckle. Methods: The HPLC was performed on an Agilent Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) using a mobile phase of acetonitrile-0.1% phosphoric acid with gradient elution at a flow rate of 1.0 mL · min -1, column temperature 30 ℃; detection wavelength 355 nm. Results: The linear ranges of chlorogenic acid, rutin and luteolin were 12.21 ~ 203.5μg.mL-1 (r = 0.9992), 2.653 ~ 132.6μg.mL-1 (r = 0.9999) and 2.794 ~ 139.7μg respectively. mL-1 (r = 0.9995). The average recoveries were 98.77% (RSD = 1.69%), 99.52% (RSD = 1.01%) and 100.84% (RSD = 1.59%), respectively. Conclusion: The method for the simultaneous determination of chlorogenic acid, rutin, quercetin and luteolin in honeysuckle content is simple, accurate, reproducible, and can provide a reliable reference for the quality control of honeysuckle medicinal materials method.