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目的 研究抑癌基因P16与喉癌的关系。方法 用免疫组化和原位杂交方法检测 18例喉癌组织中抑癌基因P16蛋白及mRNA的表达。结果 P16蛋白在喉癌、交界性肿瘤及正常喉粘膜中的检出率分别为 2 2 .2 2 %、44.44%及 6 6 .6 7% ,原位杂交表明P16mRNA在上述组织中的阳性率分别为 2 7.78%、5 5 .5 6 %及 83 .33 % ,各组分别与喉癌相比 ,均有显著性差异 (P <0 .0 5 )。并显示P16表达阳性率随喉癌病理分级的上升而降低。结论 P16在喉癌中的表达明显低于正常喉粘膜组织 ,它与喉癌的发生发展密切相关 ,可作为喉癌生物学行为的评估指标。
Objective To study the relationship between tumor suppressor gene P16 and laryngeal cancer. Methods The expression of tumor suppressor gene P16 protein and mRNA in 18 cases of laryngeal carcinoma were detected by immunohistochemistry and in situ hybridization. Results The positive rates of P16 protein in laryngeal cancer, borderline tumors and normal laryngeal mucosa were 22.22%, 44.44% and 6.6%, respectively. In situ hybridization showed that the positive rates of P16 mRNA in these tissues Respectively, 2 7.78%, 55.56% and 83.33% respectively. There were significant differences between the two groups (P <0.05). And the positive rate of P16 expression decreased with the pathological grade of laryngeal carcinoma. Conclusions The expression of P16 in laryngeal carcinoma is significantly lower than that in normal laryngeal mucosa. It is closely related to the occurrence and development of laryngeal carcinoma, which can be used as an index to evaluate the biological behavior of laryngeal carcinoma.