论文部分内容阅读
目的 本研究的目的是了解人类基因组中D10S1432及D10S12 13两个STR位点在成都汉族和甘肃东乡族群体中的遗传多态性分布及两个群体之间的关系。 方法 采用PCR、聚丙烯酰胺凝胶电泳及银染技术 ,共调查了 2 0 9例样本。 结果 在D10S1432位点上观察到 5个等位基因 ,15种基因型。在D10S12 13位点上观察到 9个等位基因 ,31种基因型。两位点的基因型频率在调查的两个群体中的分布符合Hardy -Weinberg平衡定律 (P >0 .0 5 )。经统计 ,D10S1432在这两个群体中的杂合度为 0 .6 6 4和 0 .737,个人识别几率为 0 .82 7和 0 .82 0。D10S12 13的杂合度为 0 .6 6 4和 0 .6 5 7,个人识别几率为 0 .836和 0 .882。 结论结果表明 ,D10S1432和D10S12 13两个位点在法医学个人识别和亲子鉴定中有较高应用价值。
Objectives The aim of this study was to understand the distribution of genetic polymorphisms of D10S1432 and D10S12 13 two STR loci in the Han and Gansu Dongxiang ethnic groups in the human genome and the relationship between the two populations. Methods A total of 209 samples were investigated by PCR, polyacrylamide gel electrophoresis and silver staining. Results Five alleles and 15 genotypes were observed at D10S1432 locus. Nine alleles and 31 genotypes were observed at the D10S12 13 locus. The distribution of genotype frequencies at two loci in the two populations investigated was in accordance with Hardy-Weinberg equilibrium (P> 0.05). According to the statistics, the heterozygosity of D10S1432 in these two groups is 0.664 and 0.377, and the odds of personal identification are 0.82 7 and 0.82 0. The heterozygosity of D10S12 13 was 0.664 and 0.665, and the odds of personal identification were 0.836 and 0.882. Conclusion The results showed that the two sites of D10S1432 and D10S12 13 have high value in forensic identification and paternity testing.