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采用10%聚乙二醇6000模拟干旱胁迫,研究稀土元素钕对干旱胁迫下玉米幼苗根系抗旱性的影响。结果表明,干旱胁迫显著提高根系丙二醛(MDA)含量和过氧化氢(H_2O_2)含量,提高超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,显著降低了过氧化物酶(POD)、谷胱苷肽转移酶(GST)、谷胱甘肽过氧化物酶(GPX)活性,显著降低抗坏血酸(As A)和谷胱苷肽(GSH)含量及根系生物量。干旱胁迫加剧了根系的氧化胁迫,玉米幼苗根系通过增强抗氧化酶活性抵抗干旱胁迫。外源氯化钕(NdCl_3)处理可显著提高干旱胁迫下玉米幼苗根系抗氧化酶SOD、POD、CAT、GST、GPX活性,显著提高AsA和GSH含量和根系生物量,显著降低MDA和H_2O_2含量,对APX活性无显著影响。盆栽实验结果进一步说明,NdCl_3对轻度和中度土壤干旱下玉米幼苗抗旱性均具有促进作用,对中度土壤干旱下玉米幼苗的抗旱性促进作用更佳。NdCl_3可提高玉米幼苗根系的抗旱能力,缓解干旱胁迫造成的伤害。
The effects of rare earth element neodymium on the drought resistance of maize seedling roots under drought stress were studied by using 10% polyethylene glycol 6000 to simulate drought stress. The results showed that drought stress significantly increased MDA content and H 2 O 2 content in root system, and increased the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) (POD), glutathione transpeptidase (GST) and glutathione peroxidase (GPX) activities were significantly decreased, while As A and GSH ) Content and root biomass. Drought stress exacerbated the oxidative stress of the root system, and the root system of maize seedlings was resistant to drought stress by increasing the antioxidant enzyme activity. Exogenous NdCl_3 treatment significantly increased activities of antioxidant enzymes SOD, POD, CAT, GST and GPX in roots of maize seedlings under drought stress, significantly increased AsA and GSH contents and root biomass as well as MDA and H_2O_2 contents, No significant effect on APX activity. The results of pot experiment further showed that NdCl_3 promoted the drought resistance of maize seedlings under mild and moderate soil drought and promoted the drought resistance of maize seedlings under moderate soil drought. NdCl_3 can improve the ability of drought-resistant corn seedling roots to alleviate the damage caused by drought stress.