论文部分内容阅读
目的探讨玻璃化冷冻技术应用于人成熟卵母细胞冷冻保存的临床效果。方法使用开放式载体cryotop进行人成熟卵母细胞的玻璃化冻存,对10例患者的冷冻卵子复苏后进行卵母细胞浆内单精子(ICSI)注射后行胚胎移植,观察复苏卵母细胞受精、卵裂、胚胎发育及临床妊娠情况。结果 10例患者共86枚玻璃化冻存的成熟卵母细胞复苏后77枚存活,69枚正常受精,获得65枚胚胎,共移植17枚胚胎,3例患者获临床妊娠,其中2例双胎妊娠。结论玻璃化冷冻技术是有效地保存人成熟卵母细胞的方法,有一定的临床应用价值。
Objective To investigate the clinical application of vitrification in the cryopreservation of human mature oocytes. Methods Vitrified cryopop was used to vitrify human mature oocytes. Ten frozen oocytes were resuscitated and intracytoplasmic sperm injection (ICSI) was performed in 10 patients. Embryo transfer was performed to observe the fertilization, Cleavage, embryonic development and clinical pregnancy. RESULTS: A total of 86 frozen oocytes from 10 patients survived after resuscitation, 77 survived and 69 were fertilized. Sixty-five embryos were obtained and 17 embryos were transplanted. Three of the three patients were clinically pregnant, of which two were twin pregnancies . Conclusion Vitrification is an effective method for preserving human mature oocytes and has certain clinical value.