颞骨内疱疹类病毒感染与老年性聋关系的研究

• 作 者: 戴朴 姜泗长 顾瑞 杨伟炎 袁慧军 郭维 曹菊阳 方耀云 韩东一
• 机 构: 解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉头颈外科,解放军总医院耳鼻咽喉
• 出 处: 临床耳鼻咽喉科杂志
• 发表日期: 2003年07期
• 关键词: 老年性聋 颞骨 疱疹病毒感染

目的 :探讨老年性聋患者颞骨内疱疹病毒的存在及其与老年性聋发病的关系。方法 :应用聚合酶链反应 (PCR) 酶谱法 ,仅用一对通用引物进行PCR配合酶谱分型及序列测定技术 ,检测老年性聋颞骨内 4种疱疹病毒的存在。结果 :检测出阳性对照的 4种疱疹病毒 :单纯疱疹病毒Ⅰ、Ⅱ型 (HSV 1 ,HSV 2 ) ,巨细胞病毒(CMV) ,爱泼斯坦 巴尔病毒 (EBV) ;自颞骨切片中检出HSV 1、HSV 2的存在。在老年性聋组 1 7例中 ,检出 4例疱疹病毒DNA并正确分型 ,老年对照组未检出疱疹病毒DNA。结论 :老年性聋患者颞骨内存在HSV 1和HSV 2的潜伏感染 ,其中多为HSV 1感染 ;颞骨内HSV病毒的潜伏感染可能与部分老年性聋的发生发展有关

Objective: To investigate the presence of intra-temporal herpes simplex virus in senile deafness and its relationship with senile deafness. Methods: Polymerase chain reaction (PCR) zymography was used to detect the presence of four herpesviruses in the senile deaf temporal bone by using a pair of universal primers for PCR with enzyme-linked immunosorbent assay and sequencing. Results: Four herpesviruses were detected positive for herpes simplex virus type Ⅰ and type Ⅱ (HSV 1, HSV 2), cytomegalovirus (CMV) and Epstein-Barr virus (EBV). HSV was detected from the temporal bone slices 1, the presence of HSV2. In the 17 cases of senile deafness, 4 cases of herpesvirus DNA were detected and correctly typed, while no herpesvirus DNA was detected in the elderly control group. Conclusion: There are latent infection of HSV 1 and HSV 2 in the temporal bone of senile deaf patients, most of which are HSV 1 infection. The latent infection of HSV in the temporal bone may be related to the occurrence and development of some senile deafness