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用ＵＤＧ（尿嘧啶糖化酶）防ＰＣＲ产物污染，以两个不同基因为靶序列检测同一病原菌和微孔板杂交－酶联显色检测 ＦＣＲ产物的ＵＤＰＥ（ＵＤＧ－Ｄａｐｌｅｘ ＰＣＲ－ＥＩＡ）技术，检测感染鼠疫菌植物５０份，检出阳性３５份，而常规直接培养阳性仅５份。表明ＵＤＰＥ技术灵敏度高、特异性强。
UDG (uracil glucoamylase) anti-PCR products were used to detect the contamination of the two PCR products. UDG-Daplex PCR-EIA (UDPE) was used to detect the FCR products of the same pathogen and microplate by two different genes. Infection with 50 strains of plague plants, 35 were positive, while the conventional direct culture positive only 5 copies. UDPE technology shows high sensitivity and specificity.