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目的：探讨不同年龄供体正常胃粘膜端粒长度的变化规律。方法：采用Ｓｏｕｔｈｅｒｎ杂交及图像分析技术，检测２６例７６岁以下不同年龄供体正常胃粘膜端粒限制性片段长度（Ｔｅｌｏｍｅｒｉｃｒｅｓｔｒｉｃｔｉｏｎｆｒａｇ－ｍｅｎｔ，ＴＲＦ）。结果：正常胃粘膜ＴＲＦ长度呈年龄递减趋势，６０岁以上组ＴＲＦ长度明显短于０～３９岁、４０～４９岁及５０～５９岁组（４．１±１．２ｖｓ６．４±１．８、６．０±１．０、５．５±１．１，Ｐ＜０．０５），正常胃粘膜ＴＲＦ长度与供体年龄呈明显负相关（ｒ＝－０．５６，Ｐ＝０．００２８），端粒重复序列平均每年丢失约（４１±１２）ｂｐ碱基对。结论：ＴＲＦ长度可以作为衡量胃粘膜细胞衰老的一种生物学标志
Objective: To investigate the changes of telomere length of normal gastric mucosa in different age groups. Methods: Southern blotting and image analysis were used to detect telomeric restriction fragment length (Telomeric Restriction Frag-ment, TRF) of 26 normal gastric mucosa of different ages under 76 years of age. Results: The TRF length of normal gastric mucosa showed a trend of decreasing age. The length of TRF in patients over 60 years old was significantly shorter than that of 0-39 years old, 40-49 years old and 50-59 years old group (4.1 ± 1.2 vs 6.4 ± 1.8 , 6.0 ± 1.0,5.5 ± 1.1, P <0.05). There was a negative correlation between TRF length of donor and gastric mucosa (r = -0.56, P = 0.0028 ), Telomere repeats lost on average about (41 ± 12) bp base pairs each year. Conclusion: The length of TRF can be used as a biomarker to measure the cellular senescence of gastric mucosa