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目的:考察SP825、XAD-16、LX-18、XDA-5四种大孔吸附树脂在15℃、25℃和35℃吸附知母提取液中总皂苷的热力学现象。方法:在3种不同温度和一定浓度范围内,通过树脂对知母提取液的静态吸附实验,以紫外分光光度法测定皂苷的含量并绘制吸附平衡线。吸附平衡数据用Langmuir方程和Freundich方程进行拟合,得到SP825、XAD-16、LX-18和XDA-5等大孔吸附树脂对知母总皂苷的吸附焓△H、自由能△G、吸附熵△S。结果:Langmuir方程可以描述4种树脂对知母总皂苷的吸附(R2大于0.98),平衡吸附量qm和KL随着温度的升高而减小,吸附吸附焓△H为负值,自由能△G为正值,吸附熵△S为负。结论:4种树脂吸附总皂苷过程为放热过程,低温有利于吸附,吸附为单分子层吸附,范德华力克服放热过程推动吸附主动进行。XDA-5饱和吸附量最大,吸附效果最好。
OBJECTIVE: To investigate the thermodynamics of the adsorption of total saponins in the extract of Anemarrhena asphodeloides at 15°C, 25°C and 35°C using SP825, XAD-16, LX-18 and XDA-5 macroporous adsorption resins. METHODS: The static adsorption experiments of Rhizoma Anemarrhenae extract were carried out at three different temperatures and within certain concentration ranges. The content of saponin was determined by ultraviolet spectrophotometry and the adsorption equilibrium line was drawn. Adsorption equilibrium data were fitted by Langmuir equation and Freundich equation to obtain the adsorption of total timosaponins of A. anemarrhizus with SP825, XAD-16, LX-18 and XDA-5, 焓△H, free energy △G, and adsorption entropy. △S. Results: The Langmuir equation can describe the adsorption of total timosaponins of Rhizoma anemarrhenae by four kinds of resins (R2 is greater than 0.98). The equilibrium adsorption capacities qm and KL decrease with increasing temperature. Adsorption adsorption enthalpy △H is negative and the free energy is △ G is positive and adsorption entropy ΔS is negative. Conclusion: The adsorption of total saponins from four kinds of resins is an exothermic process, low temperature is favorable for adsorption, adsorption is monolayer adsorption, and van der Waals force overcomes the exothermic process to promote adsorption. XDA-5 has the largest saturated adsorption capacity and the best adsorption effect.