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目的探讨sh-hnRNP-E2逆转录病毒对32D-P210细胞诱导的白血病模型小鼠的保护作用。方法建立32D-P210转化细胞株在C3H等基因小鼠体内定殖的慢性粒细胞白血病(Chronic myeloid leukemia,CML)模型,并进行鉴定;sh-hn-RNP-E2逆转录病毒感染32D-P210细胞24 h后,经尾静脉移植入C3H等基因雌性小鼠中,观察sh-hnRNP-E2对CML模型小鼠的保护作用。结果经鉴定已成功建立32D-P210转化细胞株在C3H等基因小鼠体内定殖的CML模型;sh-hnRNP-E2逆转录病毒预感染32D-P210靶细胞,可促进靶细胞向粒系分化,并显著延长模型小鼠的生存期。结论 sh-hnRNP-E2逆转录病毒感染对CML模型小鼠具有显著的保护效应,是一种有效的CML体内治疗策略。
Objective To investigate the protective effect of sh-hnRNP-E2 retrovirus on 32D-P210-induced leukemia model mice. Methods Chronic myeloid leukemia (CML) models of 32D-P210 cells were established in C3H-like mice and identified. Sh-hn-RNP-E2 retrovirus was used to infect 32D-P210 cells 24 h later, transplanted into C3H and other female mice via caudal vein to observe the protective effect of sh-hnRNP-E2 on CML model mice. Results The CML model of 32D-P210 transformed cell line was successfully established in C3H and other mice. The pre-infection of 32D-P210 target cells by sh-hnRNP-E2 retrovirus could promote the differentiation of target cells to granulocyte, And significantly prolong the survival of model mice. Conclusions sh-hnRNP-E2 retroviral infection has a significant protective effect on CML model mice and is an effective therapeutic strategy for CML in vivo.