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采用自然降温处理,研究稀土镧(LaCl3)对大花蕙兰组培苗耐寒性的影响,确定培养基中加入La3+的浓度,比较地上部和地下部在反映组培苗耐寒性能方面的差异性。分别测定了地上部和地下部中丙二醛(MDA)含量、膜透性、可溶性蛋白(SP)、可溶性糖(SS)、超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)7项与耐寒性有关的生理指标。结果表明,La3+提高大花蕙兰组培苗耐寒性的最适宜浓度为20 mg·L-1,浓度过高(60 mg·L-1)出现负效应。膜脂过氧化产物丙二醛(MDA)含量和细胞膜透性两项指标在反应大花蕙兰组培苗耐寒性作用明显,可作为其耐寒性鉴定指标。各浓度LaCl3处理对大花蕙兰组培苗耐寒性的提高差异明显,LaCl3对地上部和地下部耐寒性的提高在不同指标间表现出不一致性。
The effect of LaCl3 on the cold tolerance of Cymbidium hybrid seedlings was studied by natural cooling treatment. The concentration of La3 + in the medium was determined. The difference in the cold tolerance of shoots and shoots between shoots and roots was compared. . The contents of malondialdehyde (MDA), membrane permeability, soluble protein (SP), soluble sugar (SS), superoxide dismutase (SOD), peroxidase (POD) Catalase (CAT) 7 Physiological Indexes Associated with Cold Tolerance. The results showed that the optimum concentration of La3 + to improve the tolerance of Cymbidium plantlets to cold stress was 20 mg · L-1, and the highest concentration of 60 mg · L-1 was negative. Membrane lipid peroxidation products malondialdehyde (MDA) content and membrane permeability of two indicators of response Cymbidium tissue culture cold hardiness, can be used as an indicator of its cold tolerance. Different concentrations of LaCl3 treatment on the Cymbidium tissue culture cold tolerance improved significantly, LaCl3 on the ground and the lower part of the cold tolerance increased in different indicators showed inconsistencies.