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[目的]观察肝衰竭大鼠胃动力的变化并探讨其初步机制。[方法]40只Wistar大鼠随机分为肝衰竭模型组和对照组,采用葡聚糖蓝-2000为标记物观察大鼠胃排空的变化,应用乙酰胆碱酯酶(AchE)和NADPH-d组织化学染色(NOS)及肌间神经丛全层铺片技术,观察肝衰竭大鼠胃窦肌间神经丛胆碱能和氮能神经的变化,免疫组化染色观察胃窦C-kit阳性Cajal间质细胞的变化,并进行定量分析。[结果]与对照组比较,模型组大鼠胃排空明显减弱,胃窦肌间神经丛胆碱能阳性神经元数量减少、神经纤维变细、分布较稀疏,2组比较差异有统计学意义(P<0.01);模型组氮能神经阳性神经元数量及神经纤维分布明显高于对照组(P<0.01);胃窦C-kit阳性Cajal间质细胞明显少于对照组(P<0.01)。[结论]肝衰竭大鼠胃动力明显减退,其机制与胃窦肌间神经丛胆碱能神经分布减少、Cajal间质细胞减少及氮能神经分布增加有关。
[Objective] To observe the changes of gastric motility in rats with liver failure and to explore its primary mechanism. [Methods] Forty Wistar rats were randomly divided into liver failure model group and control group. The changes of gastric emptying in rats were observed by using dextran blue 2000 as the marker. AchE and NADPH-d Chemiluminescence (NOS) and myenteric plexus layer were used to observe the changes of cholinergic and nitric nerves in gastric antrum of gastric antrum in rats with liver failure. The expression of C-kit positive Cajal in gastric antrum was observed by immunohistochemical staining Changes in the quality of cells, and quantitative analysis. [Results] Compared with the control group, the gastric emptying in the model group was significantly weakened, the number of cholinergic neurons in the myenteric ganglion in the model group decreased, the nerve fibers became thinner and the distribution was more sparse, the difference between the two groups was statistically significant (P <0.01). The number of N-positive neurons and the distribution of nerve fibers in model group were significantly higher than those in control group (P <0.01). The number of C-kit positive Cajal interstitial cells in gastric antrum was significantly less than that in control group (P <0.01) . [Conclusion] The gastric motility in rats with liver failure is significantly reduced. The mechanism is related to the decrease of cholinergic nerve distribution in the antrum, the decrease of interstitial cells in Cajal and the increase of distribution of Nerve energy.