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血清中糖基转移酶和糖酶与恶性肿瘤的存在和其转移程度有关。最近报导一种半乳糖基转移酶同功酶看来像是肿瘤特异的。本文测定并比较了入类胚胎肠、结肠癌培养细胞,外科手术的结肠癌及邻近正常组织内上述酶的水平。检测了三种半乳糖基转移酶,一种N-乙酰半乳糖胺转移酶和两种唾液酸转移酶的活性,同时测定了精苷酶活性。外科手术标本置于5体积冷PBS中,于匀浆器内匀浆1分钟,再用声波处理15秒钟后离心,上述全部操作在4℃下迅速进行。培养细胞用PBS洗五次,离心后将细胞悬浮于二甲胂缓冲液内,再用声波处理,用提取液直接作糖基转移酶或糖苷酶活性测定,或制成含0.1％Triton X-100溶液,声波处理后,用于薄层等电聚焦电泳分
Serum glycosyltransferases and carbohydrases are associated with the presence of malignant tumors and their degree of metastasis. A recent report that a galactosyltransferase isozyme appears to be tumor-specific. This article determined and compared the levels of these enzymes in entero-intestinal, colon cancer-cultured cells, surgically-operated colon cancer, and adjacent normal tissues. Three galactosyltransferases, one N-acetylgalactosamine transferase and two sialyltransferases were tested for activity, and siallase activity was measured. Surgical specimens were placed in 5 vol of cold PBS, homogenized in a homogenizer for 1 minute, sonicated for 15 seconds and centrifuged, all of which were performed rapidly at 4 ° C. The cells were washed five times with PBS, centrifuged, and the cells were suspended in methhenazepine buffer and sonicated. The extract was used directly as a glycosyltransferase or glycosidase activity assay or in a solution containing 0.1% Triton X- 100 solution, after sonic treatment, for isoelectric focusing electrophoresis points