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利用诱变剂处理外植体,对其形成的愈伤组织进行了耐枯萎病菌毒素的筛选。对耐毒细胞系再生株当代及其后代进行了室内外鉴定分析。再生株的抗病性显著增加,但后代分离不符合孟德尔规律,且难以稳定。对再生株进行选择可获得抗性大为改善的单株后代。
The explants were treated with mutagen and the Fusarium oxysporum fusarium toxins were screened for callus. The indoor and outdoor identification analysis of contemporary and its offspring of the regenerative strain of drug-resistant cell lines was carried out. The resistance of the regenerated plants increased significantly, but the offspring separation did not meet the Mendelian rule and was difficult to stabilize. Selection of the regenerant plants gives offspring with improved resistance.