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旨在探究聚乙二醇修饰重组细胞珠蛋白(PEG modified recombinant cytoglobin,PEG-rCygb)对小鼠急性肝损伤的保护作用。采用CCl4诱导KM小鼠急性肝损伤模型,尾静脉注射PEG-rCygb,收集血清及肝脏组织检测各项生化指标及组织病理学变化。结果表明,PEG-rCygb治疗组小鼠肝脏系数减小,血清中AST﹑ALT水平降低,肝组织匀浆中MDA含量减少,GSH含量增加,T-SOD、CAT活性升高。肝组织切片HE染色显示PEG-rCygb可以缓解肝细胞脂肪变性,减少炎症因子,减轻肝细胞损伤。体外细胞学实验表明rCygb经PEG修饰后对H2O2造成的肝星状细胞(HSC)氧化损伤发挥的保护作用增强。研究结果显示PEG-rCygb提高了机体对自由基的清除能力,对CCl4引起的小鼠急性肝损伤具有保护作用。
To investigate the protective effect of PEG-modified recombinant cytoglobin (PEG-rCygb) on acute liver injury in mice. The acute liver injury model of KM mice was induced by CCl4, and the PEG-rCygb was injected through tail vein. The serum and liver tissues were collected to detect biochemical indexes and histopathological changes. The results showed that the liver coefficient of mice treated with PEG-rCygb decreased, the levels of AST and ALT decreased, the content of MDA decreased, the content of GSH increased and the activities of T-SOD and CAT increased. Hematoxylin-eosin staining showed that PEG-rCygb could relieve hepatic steatosis, reduce inflammatory cytokines and alleviate hepatocellular injury. In vitro cytological experiments showed that PEGylated rCygb enhanced the protective effect of H2O2 on oxidative damage of hepatic stellate cells (HSC). The results show that PEG-rCygb can improve the body’s ability to scavenge free radicals and protect CCl4-induced acute liver injury in mice.