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目的探讨病毒感染与慢性阻塞性肺病(COPD)的关系及在其发病机制中的作用。方法收集了COPD急性发作期患者86例(A组)和COPD稳定期患者32例(B组),采用生物薄片技术对患者血清进行了呼吸道合胞病毒(RSV)、腺病毒(Adv)、柯萨奇病毒(Cox)、巨细胞病毒(CMV)特异性抗体IgG、IgM检测。同时采用碱性磷酸酶———抗碱性磷酸酶(APAAP)桥联酶标法检测了T淋巴细胞亚群CD3,CD4,CD8。结果两组患者RSV,Adv,Cox和CMV特异抗体IgM,IgG阳性率比较差异无显著性(P>0.05);两组患者T淋巴细胞亚群CD3,CD4,CD8比较差异有显著性(P<0.01 or P< 0.05);在A组,病毒抗体阳性组与病毒抗体阴性组T淋巴细胞亚群CD3,CD4比较差异有显著性(P<0.01 or P<0.05),而CD8间比较差异无显著性(P>0.05)。结论病毒感染是COPD急性加重期的重要诱因,而且病毒感染与COPD发病具有相关性。
Objective To investigate the relationship between viral infection and chronic obstructive pulmonary disease (COPD) and its role in the pathogenesis. Methods Eighty-six patients (group A) with acute exacerbation of COPD and 32 patients (group B) with stable COPD were enrolled in this study. Serum samples were collected from patients with respiratory syncytial virus (RSV), adenovirus (Adv) Sox virus (Cox), cytomegalovirus (CMV) specific antibodies IgG, IgM detection. At the same time, T lymphocyte subsets CD3, CD4 and CD8 were detected by alkaline phosphatase-alkaline phosphatase (APAAP) Results The positive rate of IgM and IgG of RSV, Adv, Cox and CMV was no significant difference between the two groups (P> 0.05). There was significant difference of T lymphocyte subsets CD3, CD4 and CD8 between the two groups (P < 0.01 or P <0.05). In group A, there were significant differences in T lymphocyte subsets CD3 and CD4 between virus antibody positive group and virus antibody negative group (P <0.01 or P <0.05), but there was no significant difference between CD8 (P> 0.05). Conclusions Viral infection is an important cause of acute exacerbation of COPD, and the correlation between viral infection and the pathogenesis of COPD.