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目的:研究抑癌基因PTEN对脑胶质瘤细胞侵袭和迁移的影响。方法:用PTEN表达型质粒转染大鼠C6胶质瘤细胞,利用Matrigel体外侵袭实验和细胞迁移运动实验进行细胞侵袭和迁移的研究。结果:转染PTEN的大鼠C6胶质瘤细胞侵袭与迁移能力均明显下降。结论:PTEN在体外可以有效的抑制肿瘤细胞的侵袭和迁移过程,其具体机制还有待进一步探讨。
Objective: To investigate the effect of tumor suppressor gene PTEN on invasion and migration of glioma cells. Methods: PTEN expression plasmid was used to transfect C6 glioma cells in vitro. Matrigel invasion assay and cell migration assay were used to investigate the invasion and migration of C6 glioma cells. Results: The invasion and migration of C6 glioma cells transfected with PTEN were significantly decreased. CONCLUSION: PTEN can effectively inhibit the invasion and migration of tumor cells in vitro. The specific mechanism remains to be further explored.