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嫁接在1.5年生Rusk枳橙(C.sinensis(L)Osbeck×Poncirus trifoliata Raff)上的一年生伏令夏橙(Citrus Sinensis (L) Osbeck)盆栽树,为了提高抗寒力。在控制环境的条件下,用温度10±1℃,光照强度每平方米每秒钟500μEinsteins,相对湿度60±5%进行低温锻炼,每周一次,维持六周。伏令夏橙树按以上的条件逐渐低温锻炼,在五周后,忍受了4小时-6.7℃没有表现冻害。但是叶片直到低温锻炼五周后才没有冻害,而茎在三周后就没有冻害表现低温锻炼的第一周期间,由于碳水化合物积累的结果导致了叶片里溶质迅速地增加。脯氨酸,谷氯酸缬氨酸增加,而其它氨基酸减少,未经低温锻炼的叶片的水势量为-5巴,但低温锻炼六周后,锻炼树叶片的水势平均为-19.5巴。
Year-old pendants (Citrus Sinensis (L) Osbeck) grafted on 1.5 year old Rusk orange (C.sinensis (L) Osbeck × Poncirus trifoliata Raff) were potted in order to increase the cold resistance. Under controlled environmental conditions, exercise is performed at a temperature of 10 ± 1 ° C with a light intensity of 500 μEinsteins per second per square meter and a relative humidity of 60 ± 5% once a week for six weeks. Volt summer orange tree under the above conditions gradually low temperature exercise, after five weeks, endure 4 hours -6.7 ℃ did not show frost damage. However, the leaves did not freeze until 5 weeks of cold exercise, while the stems did not freeze after 3 weeks. During the first week of hypothermic exercise, the accumulation of carbohydrates led to a rapid increase in solute content in the leaves as a result of carbohydrate accumulation. Proline and valine glutamate increased while other amino acids decreased. The water potential of leaves without hypothermia was -5 bar, but the average water potential of exercised leaves was -19.5 bar after six weeks of hypothermic exercise.