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建立不同人参、西洋参和三七产品中人参皂苷和丙二酰基人参皂苷的高效液相色谱测定方法,为其质量评价提供实验方法和数据。方法:色谱条件:色谱柱为ProsphereC18300Acolumn(250mm×4.6mm,5μm)和ProsphereC18保护柱;流动相为乙腈-磷酸盐缓冲液梯度洗脱,流速1mL/min;A为乙腈,B为磷酸盐缓冲液(3.50gKH2PO4溶于2500mL水中,用35gK2HPO4溶于100mL水中调pH5.81);梯度洗脱程序为:0～50min,20%～33%A;50～54min,33%～100%A;54～66min,100%A;66～67min,100%～20%A;67～80min,20%A;检测波长为203nm。结果:中国人参茶中人参皂苷的含量高于韩国人参茶。3种人参提取物包括一种韩国产的和两个中国产的都未检测到人参皂苷。韩国红参根人参皂苷的含量与中国红参根相近;在8个西洋参样品中,须根样品中人参皂苷的含量高于主根,但主根之间含量无差别,与主根的粗细也无关系;三七花中人参皂苷种类与其主根中的不同,三七主根中为人参皂苷Rb1、Rg1和Rd,而三七花中为Rb1、Rb2、Rc和Rd;人参化妆品中总人参皂苷的含量较低(<0.5%),均低于其他人参产品。各种人参产品中丙二酰基人参皂苷的含量均低于人参皂苷,且多数产品中不含丙二酰基人参皂苷。结论:高效液相色谱法分离、分析人参皂苷和丙二酰基人参皂苷效果好、准确、快速、简便,可作为评价人参属植物及其产品质量的有效分析方法。
To establish a HPLC method for the determination of ginsenosides and malonyl-ginsenosides in different ginseng, American ginseng and notoginseng products, and provide experimental methods and data for their quality evaluation. Method: Chromatographic conditions: ProsphereC18300Acolumn (250 mm × 4.6 mm, 5 μm) and Prosphere C 18 guard column. The mobile phase consisted of acetonitrile-phosphate buffer gradient with a flow rate of 1 mL / min. A was acetonitrile and B was phosphate buffer (3.50gKH2PO4 dissolved in 2500mL water, 35gK2HPO4 dissolved in 100mL of water to adjust pH5.81); gradient elution program: 0 ~ 50min, 20% ~ 33% A; 50 ~ 54min, 33% ~ 100% 66 min, 100% A; 66-67 min, 100% -20% A; 67-80 min, 20% A; detection wavelength was 203 nm. Results: The content of ginsenosides in Chinese ginseng tea is higher than that of Korean ginseng tea. Three ginseng extracts, including one from South Korea and two from China, were not detected. Korean ginseng root ginsenoside content and Chinese red ginseng root similar; in the eight samples of American ginseng, the content of ginseng saponin in the fibrous root is higher than the main root, but there is no difference between the main root and root thickness; The ginsenosides in Panax notoginseng are different from those in the main roots. Panax notoginsenoside Rb1, Rg1 and Rd in Panax notoginseng, and Rb1, Rb2, Rc and Rd in Panax notoginseng are lower (Ginsenoside content in ginseng cosmetics is lower <0.5%), lower than other ginseng products. The content of malonyl ginsenosides in various ginseng products was lower than that of ginsenosides, and most products did not contain malonyl ginsenosides. Conclusion: The method of HPLC separation, analysis of ginsenosides and malonyl ginsenosides is good, accurate, rapid and simple, and can be used as an effective analytical method to evaluate the quality of ginseng plants and their products.