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目的 测定右侧前脑缺血蒙古种沙土鼠血浆降钙素基因相关肽 (CGRP)及 P物质 (SP)含量变化并观察盐酸氟桂利嗪对其影响 ,进一步探讨盐酸氟桂利嗪对缺血后神经细胞保护作用及可能机制。方法 用线扎法制作沙土鼠右侧半球前脑缺血模型 ,对各组进行神经功能缺失评分。采用放射免疫方法 (RIA)测定各组沙土鼠血浆 CGRP、SP含量。结果 药物治疗组 CGRP、SP含量与缺血对照组比较有显著差异 (P<0 .0 1) ,与假手术组与正常对照组比较没有显著差异 (P>0 .0 5 )。缺血对照组 CGRP、SP含量与假手术组及正常对照组比较有显著差异 (P<0 .0 1)。假手术组 CGRP、SP含量与正常对照组比较没有显著差异 (P>0 .0 5 )。结论 CGRP、SP参与了脑缺血的病理生理过程 ,盐酸氟桂利嗪是一种有效的钙离子拮抗剂 ,通过对 CGRP、SP含量调节 ,是其保护缺血后脑组织的一条重要途径。
Objective To determine the plasma levels of calcitonin gene related peptide (CGRP) and substance P (SP) in right forebrain Mongolian gerbils and to observe the effect of flunarizine hydrochloride on it. Neuroprotective effect of blood and its possible mechanism. Methods The rat right hemisphere forebrain ischemia model was made by the method of ligature, and the neurological deficit score of each group was scored. Radioimmunoassay (RIA) was used to determine plasma CGRP and SP contents in gerbils in each group. Results The levels of CGRP and SP in the drug-treated group were significantly different from those in the ischemic control group (P <0.01). There was no significant difference between the sham operation group and the normal control group (P> 0.05). The levels of CGRP and SP in ischemic control group were significantly different from those in sham operation group and normal control group (P <0.01). Sham operation group CGRP, SP content compared with the normal control group no significant difference (P> 0.05). Conclusion CGRP and SP are involved in the pathophysiological process of cerebral ischemia. Flunarizine hydrochloride is an effective calcium antagonist. It regulates CGRP and SP, which is an important way to protect cerebral ischemia.