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在大鼠肢体缺血模型上观察了质粒pcDNA3对缺血骨骼肌肌浆网 (SR)Ca2 + 转运的影响。结果显示 ,骨骼肌缺血时SRCa2 + 转运 (Ca2 + 摄入与释放 )较非缺血肌肉增强 ,而质粒pcDNA3与SR上DNA结合蛋白结合之后 ,可进一步增强缺血骨骼肌SRCa2 + 摄入 (P <0 0 1)及释放速率 (P <0 0 5 )。提示质粒DNA对正常及缺血大鼠骨骼肌的SRCa2 + 转运能力均有影响 ,其临床病理生理意义值得进一步研究。
The effect of plasmid pcDNA3 on the Ca2 + transport in sarcoplasmic reticulum (SR) of ischemic skeletal muscle was observed in a rat model of limb ischemia. The results showed that SRCa2 + transport (Ca2 + uptake and release) during skeletal muscle ischemia was stronger than non-ischemic muscle, and plasmid pcDNA3 combined with DNA binding protein on SR could further enhance the uptake of SRCa2 + P <0 01) and release rate (P 0 05). These results suggest that plasmid DNA has an effect on SRCa2 + transport ability in skeletal muscle of normal and ischemic rats, and its clinical pathophysiological significance deserves further study.