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感染性休克的特征是心输出量正常或升高,全身血管阻力降低。持续性的血管扩张是感染性休克病人的常见死亡原因。但是造成血管扩张的机理尚不清楚。作者利用大鼠主动脉环模型,研究脓毒症血清对血管张力的影响,以验证血管扩张物质降低全身血管阻力的假说。选用雄性S-D大鼠。取其4mm宽的主动脉环,两侧以钩子固定,浸泡在Krebs-Ringer缓冲液内。用去甲肾上腺素和乙酰胆碱处理主动脉环使其收缩和舒张,以检查血管内皮细胞
Septic shock is characterized by normal or elevated cardiac output and decreased systemic vascular resistance. Sustained vasodilation is a common cause of death in septic shock patients. However, the mechanism of vasodilation is not clear. The authors used a rat aortic annulus model to study the effect of sepsis serum on vascular tone to verify the hypothesis that vasodilators reduce systemic vascular resistance. Male S-D rats were used. Take a 4 mm wide aortic ring, hooked on both sides and dipped in Krebs-Ringer’s buffer. Aortic rings were treated with norepinephrine and acetylcholine to cause systole and diastole to examine vascular endothelial cells