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利用RAPD技术对香蕉 (MusananaLour.) 3 3个品种的遗传变异进行了研究。从 2 4 9个随机引物中筛选出18个有效引物 ,用它们共扩增出 192条DNA带 ,其中 183条为多态性带 ,占 95 .3 1% ,平均每个引物扩增的DNA带数为 10 .67条。利用 18个有效引物扩增的 192条DNA带对香蕉 3 3个品种间的亲缘关系进行UPGMA聚类分析 ,计算出 3 3个品种间的平均遗传距离为 0 .3 4 12。在此基础上建立了香蕉 3 3个品种的DNA分子系统树状图。该系统将香蕉 3 3个品种划归A、B、C和D 4个群 ,其中A群 2 0个品种 ,B群 5个品种 ,C群 2个品种 ,D群 6个品种 ;A群又可以分为 3个亚群。对香蕉遗传多样性分子基础进行了探讨。
The genetic variations of 3 varieties of banana (MusananaLour.) Were studied by RAPD technique. Eighteen valid primers were screened from 294 random primers, and a total of 192 DNA bands were amplified with them, of which 183 were polymorphic bands (95.31%). The average DNA amplified by each primer The number of bands is 10.67. Using UPGMA cluster analysis of 192 DNA bands amplified by 18 effective primers, we found that the average genetic distance among the 3 cultivars was 0.3412. On this basis, a DNA molecular phylogenetic tree was constructed for 33 varieties of banana. The system divided 3 3 varieties of banana into 4 groups A, B, C and D, including 20 varieties of A group, 5 varieties of B group, 2 varieties of C group and 6 varieties of D group; Can be divided into 3 subgroups. The molecular basis of banana genetic diversity was discussed.