论文部分内容阅读
为探讨基因的改变在膀胱移行细胞癌发生过程中的意义，应用聚合酶链反应——限制性片段长度多肽性（ＰＣＲ－ＲＦＬＰ）法，检测５０例膀胱移行细胞癌ｒａｓ、ｐ５３基因突变。结果显示：正常膀胱粘膜未检测到基因突变，膀胱移行细胞癌Ｈａ－ｒａｓ基因第１２位密码子突变为１６例（３２％），ｐ５３基因突变为９例（１８％），均系２４８位点突变。Ｈａ－ｒａｓ－１２位点突变率随病理分级增高而增高，与膀胱移行细胞癌分级显著相关（Ｐ＜０．０５）。ｒａｓ基因、ｐ５３基因突变患者死亡率高于无突变患者。４例膀胱移行细胞癌存在ｒａｓ基因及ｐ５３基因共同突变。
To investigate the significance of gene changes in transitional cell carcinoma of bladder, the mutations of ras and p53 in 50 cases of bladder transitional cell carcinoma were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that no gene mutation was detected in normal bladder mucosa. The codon 12 mutation of Ha-ras gene in bladder transitional cell carcinoma was 16 (32%) and the mutation of p53 gene was 9 (18%) in 248 cases mutation. The mutation rate of Ha-ras-12 locus increased with the increase of pathological grade, and was significantly correlated with the grade of bladder transitional cell carcinoma (P <0.05). ras gene, p53 mutations in patients with higher mortality than non-mutant patients. Four cases of bladder transitional cell carcinoma ras gene and p53 gene co-mutation.