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目的:探讨宫内发育迟缓(intrauterine growth retardation,IUGR)大鼠在胚胎期和新生后Insulin/FoxO1/Pdx1/MafA基因的表达。方法:清洁级SD大鼠,雌雄鼠1∶3合笼交配,孕鼠按受孕顺序随机分为2组(IUGR组和对照组)。IUGR组自妊娠第1天至分娩给予正常对照组饲料进食量的半量;对照组妊娠全程任意摄取饲料;应用显微分离及提取技术取大鼠胚胎发育第14.5天、第19.5天及新生大鼠胰腺组织;采用HE染色,光镜观察胰腺不同发育时期形态学变化;使用RT-PCR技术检测胚胎发育第14.5天,第19.5天及新生大鼠胰腺Insulin/FoxO1/Pdx1/MafA基因的表达情况。结果:①与对照组大鼠相比,IUGR组胰腺在发育各期均有形态学上的改变,表现为组织松散,结构不清晰,胰岛数量及面积减少;②Insulin/FoxO1/Pdx1/MafA基因全程均有表达;③Insulin、MafA基因随胎龄的增长表达量增多,与对照组相比,IUGR组Insulin基因表达无明显差异(P>0.05),而MafA基因表达较之减少(P<0.05);④FoxO1、Pdx1基因均在胚胎早期表达较多,随胎龄的增长表达量下降,且与对照组相比,IUGR组Pdx1基因表达无明显差异(P>0.05),FoxO1表达较对照组减少(P<0.05)。结论:宫内发育迟缓对胰腺发育相关基因Insulin/FoxO1/Pdx1/MafA的表达有影响。
Objective: To investigate the expression of Insulin / FoxO1 / Pdx1 / MafA gene in embryonic and neonatal rats after intrauterine growth retardation (IUGR). Methods: Cleansing grade SD rats were mated with male and female mice 1: 3 and pregnant rats were randomly divided into 2 groups (IUGR group and control group) according to the order of pregnancy. IUGR group from the first day of gestation to childbirth to the normal control group, half the amount of feed intake; the control group throughout the pregnancy intact feed; application of microscopic separation and extraction technology to take rat embryonic day 14.5, day 19.5 and neonatal rats The morphological changes of pancreas at different developmental stages were observed by light microscopy with HE staining. The expression of Insulin / FoxO1 / Pdx1 / MafA in pancreas was detected by RT-PCR at day 14.5 and day 19.5. Results: ① Compared with the control group, the pancreas of IUGR group showed morphological changes in all stages of development, showing loosely organized, unclear structures and decreased islet number and area; ② The full-length of Insulin / FoxO1 / Pdx1 / MafA gene (P <0.05); (3) The expression of Insulin and MafA increased with the increase of gestational age. Compared with the control group, the expression of Insulin gene in IUGR group was not significantly different (P> 0.05); ④The expression of FoxO1 and Pdx1 genes in the early embryo was higher than that in the control group (P <0.05), and the expression of FoxO1 in IUGR group was significantly lower than that in the control group (P> 0.05) <0.05). Conclusion: Intrauterine growth retardation has an effect on the expression of Insulin / FoxO1 / Pdx1 / MafA in pancreas.