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目的 :应用分子生物学的实验方法和技术研究中长跑运动员胃肠菌群区系结构分布特征。方法 :直接从运动员粪便样品中提取细菌总DNA ,并以此为模板进行PCR扩增 ,得到不同运动个体胃肠细菌基因指纹图 ;然后将 1名运动员的PCR产物用地高辛标记为探针 ,与其余 6名运动个体的胃肠细菌指纹图进行核酸印迹杂交 ;最后对目的基因片段进行克隆和测序。结果 :(1)不同运动个体胃肠菌群中既存在共有菌群 ,又存在个体特征菌群 ;(2 )普遍存在于运动员胃肠道中的某种特征菌群 ,在基因库中无与其同源的序列 ,可能是一种尚未被报道的新型菌群。
OBJECTIVE: To study the distribution of the flora of gastrointestinal flora of middle and long distance runners using molecular biology experimental methods and techniques. Methods: Total bacterial DNA was extracted directly from the stool samples of the athletes. PCR amplification was carried out using this template as a template to obtain the fingerprints of gastrointestinal bacterial genes in different sports individuals. Then, a PCR product of one athlete was labeled with digoxigenin And the other six sports individuals gastrointestinal bacterial fingerprinting for nucleic acid blot hybridization; finally the target gene fragments were cloned and sequenced. Results: (1) There are both common flora and individual characteristic flora in the gastrointestinal flora of different sports individuals; (2) Some characteristic flora commonly found in the gastrointestinal tract of athletes does not exist in the gene pool The source sequence may be a new type of flora that has not yet been reported.