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目的:观察重组人表皮细胞生长因子等多种细胞因子对滋养层细胞表达基质金属蛋白酶-2,9的影响。方法:将分离纯化的原代培养早孕滋养层细胞分别给予10 ng/mL重组人表皮细胞生长因子、白细胞介素-1α,10及5ng/mL肿瘤坏死因子-α处理48 h后,采用RT-PCR方法检测基质金属蛋白酶-2和9的转录表达。结果:重组人表皮细胞生长因子、肿瘤坏死因子-α增强滋养层细胞中基质金属蛋白酶-2的表达,而白细胞介素-1α,10则对早孕滋养层细胞中基质金属蛋白酶-2的表达具有抑制作用;重组人表皮细胞生长因子亦增强滋养层细胞中基质金属蛋白酶-9的表达,白细胞介素-10对早孕滋养层细胞中基质金属蛋白酶-9的表达同样具有抑制作用,而肿瘤坏死因子-α则对早孕滋养层细胞中基质金属蛋白酶-9的表达无影响。结论:重组人表皮细胞生长因子可通过诱导基质金属蛋白酶-2,9的表达对滋养层细胞侵入起促进作用;白细胞介素-10则通过抑制基质金属蛋白酶-2,9的表达对滋养层细胞的侵袭性起负调节作用。肿瘤坏死因子-α和白细胞介素-1α亦可能通过对不同类型基质金属蛋白酶表达的影响参与滋养层细胞的侵入调节。
Objective: To observe the effect of recombinant human epidermal growth factor (EGF) and other cytokines on the expression of matrix metalloproteinase-2 and -9 in trophoblast cells. Methods: The primary cultured trophoblast cells were treated with 10 ng / mL recombinant human epidermal growth factor, interleukin-1 alpha, 10 and 5 ng / mL tumor necrosis factor-alpha for 48 h, respectively. RT- PCR method to detect the transcriptional expression of matrix metalloproteinase-2 and 9. Results: Recombinant human epidermal growth factor and tumor necrosis factor-α enhanced the expression of matrix metalloproteinase-2 in trophoblast cells, whereas interleukin-1α and 10 had the expression of matrix metalloproteinase-2 Inhibition; recombinant human epidermal growth factor also enhances the expression of matrix metalloproteinase-9 in trophoblast cells, and interleukin-10 also inhibits the expression of matrix metalloproteinase-9 in trophoblast cells in early pregnancy, whereas tumor necrosis factor -α had no effect on the expression of matrix metalloproteinase-9 in trophoblast cells in early pregnancy. Conclusion: Recombinant human epidermal growth factor can promote the invasion of trophoblast cells by inducing the expression of matrix metalloproteinase-2 and -9. Interleukin-10 inhibits the invasion of trophoblast cells by inhibiting the expression of matrix metalloproteinase-2 and -9 The aggressiveness plays a negative regulatory role. Tumor necrosis factor-alpha and interleukin-1 alpha may also be involved in the regulation of invasion by trophoblast cells by affecting the expression of different types of matrix metalloproteinases.